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36B, C)

The RNA and primers were then annealed by incubating as follows: 90 C (1 min), 80 C (1 min), 70 C (1 min), 60 C (1 min), 50 C (1 min), and 40 C (6 min)

Understanding this pathway is not only key to elucidating the origin of SFN but also provides the essential toolkit for its sustainable enhancement through genetic biofortification

By inducing mild oxidative stress, they can activate endogenous defense systems, particularly the Nrf2 pathway, which plays a central role in regulating antioxidant responses